Bacteriophages Methods and Protocols 1st edition by Ebenezer Tumban 1071635484 ‎978-1071635483

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ISBN-10 ‏ : ‎ 1071635484

ISBN-13 ‏ : ‎ 978-1071635483

Author:  Ebenezer Tumban

This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular techniques, protocols for isolation, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls.

Bacteriophages Methods and Protocols 1st Table of contents:

1. Structural and genomic diversity of bacteriophages.
2. The diversity of bacteriophages in human gut.
3. Breaking the ice
4. A review of phages from polar ecosystems.
5. The diversity of bacteriophages in hot spring.
6. Isolation of bacteriophage from soil samples in a poorly equipped field lab in Kruger National Park.
7. Purification and up-concentration of bacteriophages and viruses from fecal samples.
8. Isolation of enterococcus phages from municipal wastewater samples using an enrichment step.
9. Phage DNA extraction, genome assembly, and genome closure.
10. Enumeration of bacteriophages by plaque assay.
11. Detection and quantification of phages in wastewater samples by culture and molecular methods.
12. Flow virometry: A fluorescence-based approach to enumerate bacteriophages in liquid samples.
13. A metagenomics approach to enumerate bacteriophages in a Food Niche.
14. Bioinformatic analysis of Staphylococcus phages: a key step for safe cocktail development.

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