Quantitative biological and clinical mass spectrometry an introduction 1st Edition by Anthony Mallet 1119281214 9781119281214

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ISBN-10 :  1119281214 

ISBN-13 :  9781119281214

Author:  Anthony I. Mallet 

A basic overview of mass spectrometry relevant to life and health science applications, illustrated throughout with relevant case studies

This introductory text provides information and assistance to new users of mass spectrometry (MS) working in clinical or biochemical fields who are faced with implementing and designing quantitative mass spectrometric assays for a variety of classes of molecules of biological interest. It presents a detailed discussion on how to optimize measurement parameters for a candidate reference quantitative analysis, including calibration procedures, sensitivity, reproducibility, speed of assay and compliance with regulatory authorities. 

Quantitative biological and clinical mass spectrometry : an introduction 1st Table of contents:

1 The Instrument: Ion Creation
1.1 Introduction
1.2 Sample handling
1.3 Vacuum ion sources
1.4 Atmospheric pressure ion sources
1.5 Ambient ionisation methods
References
2 The Instrument
2.1 The analyser
2.2 Tandem mass spectrometry
2.3 The detector
2.4 Control and data handling
2.5 Ambient ionisation
2.6 Summary
References
3 The Mass Spectrum
3.1 Spectral output
3.2 Electron ionisation/chemical ionisation spectra
3.3 Stable isotopes and accurate m/z determinations
3.4 Chemical ionisation
3.5 Atmospheric‐pressure spray ionisation
3.6 Tandem mass spectra, MS/MS
3.7 Manipulating chromatographic data output
3.8 Fragmentation of even‐ and odd‐electron ions
3.9 Spectra of peptides proteins and other biopolymers
3.10 Summary
References
4 Sample Handling Prior to Ionisation
4.1 Gas chromatography
4.2 Liquid chromatography: HPLC/UHPLC
4.3 Alternative sample purification methods
4.4 Theory of chromatography relevant to clinical MS ion sources
4.5 Avoiding chromatography: flow injection analysis
4.6 Summary
References
5 Establishing Optimum Specificity
5.1 Structure from the molecular ion or its derivative
5.2 Structure from fragmentation
5.3 Spectra of peptides and proteins
5.4 Example of the deduction of the identity of an unknown
5.5 Potential problems with MS/MS for quantitative analysis
5.6 Conclusions
References
6 Quantitative Analysis with Mass Spectrometry
6.1 Introduction
6.2 Calibration with internal standards
6.3 Creation of a calibration curve
6.4 Assay validation
6.5 Matrix interference
6.6 Immediate calibrations
6.7 Selected or multiple ion recording
6.8 Summary
References
7 Examples of Quantitative Analysis
7.1 Vitamin D metabolite analysis
7.2 Testosterone/epitestosterone
7.3 Oxygenated neural sterols
7.4 Cholic acids
7.5 Phospholipids
7.6 8‐iso‐Prostaglandin F2α
7.7 Metanephrine and normetanephrine
7.8 Isotopic internal calibration assay for clozapine and norclozapine
7.9 Glycolipids and carbohydrates
7.10 Matrix‐assisted laser desorption ionisation analysis of simple carbohydrates
7.11 LC–MS/MS ceramides in Fabry disease
7.12 N‐Tetrasaccharides from protein glycosylation defects
7.13 Peptides
7.14 Hepcidin
7.15 Thyroglobulin
7.16 Quantitative proteomics
7.17 Summary
References
8 Rapid Clinical Analysis
8.1 Flow injection analysis
8.2 Dried blood spots and neonate inborn errors of metabolism analysis
8.3 Haemoglobin analyses
8.4 Application of ambient ionisation methods
8.5 Conclusions

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