Bacterial Persistence Methods and Protocols 1st Edition by Jan Michiels 1493928538 9781493928538

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Product details:

ISBN-10 : 1493928538 

ISBN-13 : 9781493928538

Author:  Jan Michiels 

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Bacterial Persistence Methods and Protocols 1st Table of contents:

1. A Historical Perspective on Bacterial Persistence.- Persisters
2. Methods for Isolation and Identifying Contributing Factors: A Review.
3.  A General Method for Measuring Persister Levels in Escherichia Coli Cultures.
4. Optimized Method for Measuring Persistence in Escherichia cCli with Improved Reproducibility.
5. A Micro plate-Based System as In Vitro Model of Biofilm Growth and Quantification.
6. Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms.
7. Quantitative Measurements of Type I and Type II Persisters Using ScanLag.
8. Analyzing Persister Physiology with Fluorescence-Activated Cell Sorting.
9. Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.

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Tags: Bacterial Persistence, Methods, Protocols, Jan Michiels