BCL 2 Family Proteins Methods and Protocols 1st edition by Evripidis Gavathiotis 1493988603 9781493988600

BCL-2 Family Proteins: Methods and Protocols 1st edition by Evripidis Gavathiotis – Ebook PDF Instant Download/DeliveryISBN:  1493988603, 9781493988600 

Full download BCL-2 Family Proteins: Methods and Protocols 1st edition after payment.

Product details:

ISBN-10 :  1493988603 

ISBN-13 :  9781493988600

Author:  Evripidis Gavathiotis 

This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to specific BCL-2 family proteins; flow-cytometry-based methods for measuring BCL-2 proteins and mitochondrial-based cell death; measuring activity and interactions of BCL-2 family proteins in the presence of mitochondria, artificial membranes or yeast; conformational activation and oligomerization of pro-apoptotic proteins BAX and BAK leading to cytochrome c release and apoptosis; structural and biophysical studies in solution and lipid vesicles using nuclear magnetic resonance, cryo-electron microscopy, fluorescence microscopy and electron paramagnetic resonance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

BCL-2 Family Proteins: Methods and Protocols 1st Table of contents:

1. Overview of BCL-2 Family Proteins and Therapeutic Potentials 

2. Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins 

3. Application of Mito-Priming to Generate BCL-2 Addicted Cells 

4. BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies 

5. Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP) 

6. Investigating BCL-2 Family Protein Interactions in Yeast 

7. Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family 

8. Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP 

9. Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family 

10. Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol 

11. Quantification of BCL-2 Family Members by Flow Cytometry 

12. Methods to Probe Calcium Regulation by BCL-2 FamilyMembers 

13. Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK 

14. Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage 

15. BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy 

16. Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs 

17. Cryo-Electron Microscopy to Study Bax Pores and MOMP 

18. CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions.

 

People also search for BCL-2 Family Proteins: Methods and Protocols 1st:

bcl-2 family proteins and cancer
    
bcl-2 family proteins in apoptosis
    
bcl-2 family proteins
    
bcl 2 family proteins
    
bcl2 family of proteins

 

Tags: Family Proteins, Methods, Protocols, Evripidis Gavathiotis