Biotechnologies for Plant Mutation Breeding Protocols 1st Edition by Joanna Jankowicz Cieslak, Thomas Tai, Jochen Kumlehn, Bradley Till 3319450193 9783319450193

Biotechnologies for Plant Mutation Breeding: Protocols 1st Edition by Joanna Jankowicz-Cieslak, Thomas H. Tai, Jochen Kumlehn, Bradley J. Till – Ebook PDF Instant Download/DeliveryISBN:  3319450193, 9783319450193 

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Product details:

ISBN-10 :  3319450193 

ISBN-13 :  9783319450193

Author:   Joanna Jankowicz-Cieslak, Thomas H. Tai, Jochen Kumlehn, Bradley J. Till 

This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

 

Biotechnologies for Plant Mutation Breeding: Protocols 1st Table of contents:

1. Front Matter

2. Introduction

Mutation Induction and Chimera Dissociation 

3. Chemical and Physical Mutagenesis in Jatropha curcas

4. Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana 

5. Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.) 

6. Optimisation of Somatic Embryogenesis in Cassava 

7. Creation of a TILLING Population in Barley after Chemical Mutagenesis with Sodium Azide and MNU

8. Site‑Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen 

9. Doubled Haploidy as a Tool for Chimera Dissolution of TALEN‑Induced Mutations in Barley 

Phenotypic Screening

10. Field Evaluation of Mutagenized Rice Material 

11. Root Phenotyping Pipeline for Cereal Plants 

12. Breeding New Aromatic Rice with High Iron using Gamma Radiation and Hybridization 

13. Utilising NIRS for Qualitative and Non‑Destructive Identification of Seed Mutants in Large Populations 

14. Proteome Analyses of Jatropha curcas 

Genotypic Screening 

15. Low‑Cost Methods for DNA Extraction and Quantification 

16. A Protocol for Benchtop Extraction of Single‑Strand‑Specific Nucleases for Mutation Discovery 

17. Protocol for Validation of Doubled Haploid Plants by Enzymatic Mismatch Cleavage 

18. Bioinformatics‑Based Assessment of the Relevance of Candidate Genes for Mutation Discovery

19. Mutation Detection by Analysis of DNA Heteroduplexes in TILLING Populations of Diploid Species 

20. Determining Mutation Density using Restriction Enzyme Sequence Comparative Analysis 

21. Next‑Generation Sequencing for Targeted Discovery of Rare Mutations in Rice

 

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Tags: Biotechnologies, Plant Mutation, Protocols, Joanna Jankowicz Cieslak, Thomas Tai, Jochen Kumlehn, Bradley Till