Cell Based Assays Using iPSCs for Drug Development and Testing 1st edition by Carl Fredrik Mandenius, James Ross 1493994762 9781493994762

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ISBN-10 :  1493994762 

ISBN-13 :  9781493994762

Author: Carl-Fredrik Mandenius, James A. Ross 

The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.

Cell Based Assays Using iPSCs for Drug Development and Testing 1st Table of contents:

1. Cell-Based Assays Using Differentiated Human Induced Pluripotent Cells
2. Differentiation of iPC to Target Cells for Assays
3. Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells
4. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling
5. Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation
6. Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies
7. Improved Protocol for Cardiac Differentiation and Maturation of Pluripotent Stem Cells
8. Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors
9. A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes
10. Differentiation of hiPS Cells into Definitive Endoderm for High-Throughput Screening
11. High Throughput Metabolomics and Proteomic Cell-Based Assays
12. LC-MS/MS-Based Metabolomics for Cell Cultures
13. Preparation of iPSCs for Targeted Proteomic Analysis
14. Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes
15. Mass Spectrometry Measurement of Albumin–Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation
16. High Content Imaging
17. Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates
18. High-Content Autophagy Analysis in iPSC-Derived Neurons Using Immunofluorescence
19. High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons

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Tags: Cell Based Assays, Using iPSCs, Drug Development, Testing, Carl Fredrik Mandenius, James Ross