Phytoplasmas: Methods and Protocols 1st edition by Rita Musetti, Laura Pagliari 1493988365 9781493988365

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Product details:

ISBN-10 :  1493988365 

ISBN-13 :  9781493988365

Author:  Rita Musetti, Laura Pagliari 

This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Phytoplasmas: Methods and Protocols 1st Table of contents:

1. The Phytoplasmas
2. An Introduction
3. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus.
4. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture.
5. Dodder Transmission of Phytoplasmas.- Insect Maintenance and Transmission.
6. Capturing Insect Vectors of Phytoplasmas.- Insect Vector Transmission Assays.
7. Molecular Identification of Phytoplasma Vector Species.
8. Dienes’ Staining and Light Microscopy for Phytoplasma Visualization.
9. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas.
10. Visualisation of Phytoplasmas using Electron Microscopy.
11. Automated DNA Extraction for Large Numbers of Plant Samples.
12. DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts.
13. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene.
14. PCR and RFLP Analyses Based on the Ribosomal Protein Operon.

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