Plant Proteomics Methods and Protocols Methods in Molecular Biology 2139 3rd Edition by Jesus V. Jorrin-Novo, Luis Valledor, Mari Angeles Castillejo, Maria-Dolores Rey – Ebook PDF Instant Download/DeliveryISBN: 1071605275, 9781071605271
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Product details:
ISBN-10 : 1071605275
ISBN-13 : 9781071605271
Author: Jesus V. Jorrin-Novo, Luis Valledor, Mari Angeles Castillejo, Maria-Dolores Rey
This third edition detailsrecent advances made in the field of proteomics and the application to plant biology and translational research. In recent years, improvements have been made to protocols on high-throughput proteomics at all workflow stages, from wet to dry labs. Chapters are divided into nine parts, covering methods and protocols such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, study and analysis of PTMs, protein interactions, specific families of proteins, and finally proteomics in translational research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Plant Proteomics Methods and Protocols Methods in Molecular Biology 2139 3rd Table of contents:
- What Is New in (Plant) Proteomics Methods and Protocols: The 2015–2019 Quinquennium
- Multiple Biomolecule Isolation Protocol Compatible with Mass Spectrometry and Other High-Throughput Analyses in Microalgae
- Protein Interaction Networks: Functional and Statistical Approaches
- Specific Protein Database Creation from Transcriptomics Data in Nonmodel Species: Holm Oak
- Subcellular Proteomics in Conifers: Purification of Nuclei and Chloroplast Proteomes
- Apoplastic Fluid Preparation from Arabidopsis thaliana Leaves Upon Interaction with a Nonadapted Powdery Mildew Pathogen
- Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS
- A Protocol for the Plasma Membrane Proteome Analysis of Rice Leaves
- Isolation, Purity Assessment, and Proteomic Analysis of Endoplasmic Reticulum
- Dimethyl Labeling-Based Quantitative Proteomics of Recalcitrant Cocoa Pod Tissue
- Quantitative Profiling of Protein Abundance and Phosphorylation State in Plant Tissues Using Tandem Mass Tags
- Optimizing Shotgun Proteomics Analysis for a Confident Protein Identification and Quantitation in Orphan Plant Species: The Case of Holm Oak
- Combining Targeted and Untargeted Data Acquisition to Enhance Quantitative Plant Proteomics Experiments
- A Phosphoproteomic Analysis Pipeline for Peels of Tropical Fruits
- Label-Free Quantitative Phosphoproteomics for Algae
- Targeted Quantification of Phosphopeptides by Parallel Reaction Monitoring
- Enrichment of N-Linked Glycopeptides and Their Identification by Complementary Fragmentation Techniques
- High-Resolution Lysine Acetylome Profiling by Offline Fractionation and Immunoprecipitation
- A Versatile Workflow for the Identification of Protein–Protein Interactions Using GFP-Trap Beads and Mass Spectrometry-Based Label-Free Quantification
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Tags: Plant Proteomics, Protocols Methods, Molecular Biology, Jesus Jorrin Novo, Luis Valledor, Mari Angeles Castillejo, Maria Dolores Rey


