PROTEIN DOWNSTREAM PROCESSING design development and application of high 2nd Edition by Nikolaos Labrou ISBN 978-1071607749 107160774X

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Product details: 

ISBN 10:  107160774X

ISBN 13:  978-1071607749

Author: Nikolaos E. Labrou
This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry.

Table of contents: 

PART I SCREENING AND DESIGN PURIFICATION STRATEGIES

1 Protein Purification Technologies.

Nikolaos E. Labrou

2 High-Throughput Process Development: I-Process Chromatography.

Anurag S. Rathore and R. Bhambure

3 High-Throughput Process Development: II-Membrane Chromatography

Anurag S. Rathore and S. Muthukumar

4 Media Selection in Ion Exchange Chromatography in a Single Microplate Charlotte Cabanne and Xavier Santarelli

5 High-Throughput Screening of Dye-Ligands for Chromatography Sunil Kumar and Narayan S. Punekar

6 Technical Optimization for the High-Throughput Purification of Antibodies on Automated Liquid Handlers..

Peter M. Schmidt

PART II LOW-RESOLUTION PROTEIN PURIFICATION METHODS

7 Aqueous Two-Phase Systems for Cleanup and Recovery of Enzymes from Plants and Plant-Derived Extracts..

Oscar Aguilar, Erick Heredia-Olea, Esther Perez-Carrillo, and Marco Rito-Palomares

8 Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins……. Anurag S. Rathore and R. Bhambure

9 Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments

Marco’s Ario

PART III HIGH-RESOLUTION PROTEIN PURIFICATION METHODS

10 Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

Ana Sofia Pina, Iris L. Batalha, Ana M. G. C. Dias, and Ana Cecília A. Roque

11 Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins

Sharon Williams, Phil Morton, and Dev Baines

My Hedhammar, Johan Nilvebrant, and Sophia Hober

13 An Orthogonal Fusion Tag for Efficient Protein Purification.. Johan Nilvebrant, Mikael Astrand, and Sophia Hober

14 Biomimetic Affinity Ligands for Protein Purification Isabel T. Sousa and M. Angela Taipa

15 Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification.

Evangelia G. Chronopoulou, Georgios Premetis, Christina Varotsou, Nikolaos Georgakis, Elisavet Ioannou, and Nikolaos E. Labrou

16 Design of Affinity Chromatography Peptide Ligands Through Combinatorial Peptide Library Screening

G. R. Barredo, S. L. Saavedra, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, O. Cascone, and S. A. Camperi

17 Zca: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification

Julia Scheffel, Sara Kanje, and Sophia Hober

18 Macroporous Polymer Monoliths for Affinity Chromatography and Solid-Phase Enzyme Processing..

E. G. Korzhikova-Vlakh, G. A. Platonova, and T. B. Tennikova

19 Sample Displacement Batch Chromatography of Proteins.

Laura Heikaus, Siti Hidayah, Manasia Gaikwad, Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter

20 Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3

Salvatore G. De-Simone and David W. Provance Jr.

21 Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins..

Sanhita Maity, Musaab Al-Ameer, Ravi Kumar Gundampati, Shilpi Agrawal, and Thallapuranam Krishnaswamy Suresh Kumar

22 Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+.

Jessica J. Gomez-Lugo, Bryan D. Santos, David A. Perez-Perez, Jorge M. Montfort-Gardeazabal, Megan M. McEvoy, and Xristo Zarate

23 Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin.

Tao Chen, Gaili Guo, Guoqing Tan, Ying Wang, and Yifeng Li

PART IV ASSESSING PROTEIN STRUCTURAL INTEGRITY, PURITY, AND STABILIZATION

24 Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry.

Cosima D. Calvano, Mariachiara Bianco, Ilario Losito, and Tommaso R. I. Cataldi

25 Protein Structure Analysis and Validation with X-Ray Crystallography.

Anastassios C. Papageorgiou, Nirmal Poudel, and Jesse Mattsson

26 Measuring Binding Constants of His-Tagged Proteins Using Affinity Chromatography and Ni-NTA Immobilized Enzymes.

Annette C. Moser, Benjamin White, and Frank A. Kovacs

27 Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.

Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda

Dráberová

PART V APPLICATIONS/CASE STUDIES

28 G-Protein-Coupled Receptor Expression and Purification

Karolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos

29 (Hyper) Thermophilic Enzymes: Production and Purification.

Pierpaolo Falcicchio, Mark Levisson, Servé W. M. Kengen, Sotirios

Koutsopoulos, and John van der Oost

30 Screening of Recombinant Lignocellulolytic Enzymes Through Rapid Plate Assays

Anthi Karnaouri, Anastasia Zerva, Paul Christakopoulos, and Evangelos Topakas

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