The ABCs of Gene Cloning Dominic 3rd edition W. S. Wong 3319779826 9783319779829

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Product details:

• ISBN 10: 3319779826
• ISBN 13: 9783319779829
• Author: W. S. Wong

Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas.  The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.

Table of contents:

1. Introductory Concepts

2. Structures of Nucleic Acids

3. Structures of Proteins

4. The Genetic Process

5. Organization of Genes

6. Reading the Nucleotide Sequence of a Gene

Part Two. Techniques and Strategies of Gene Cloning

7. Enzymes Used in Cloning

8. Techniques Used in Cloning

9. Cloning Vectors for Introducing Genes into Host Cells

10. Gene-Vector Construction

11. Transformation

12. Isolating Genes for Cloning

Part Three. Impact of Gene Cloning: Applications in Agriculture

13. Improving Tomato Quality by Antisense RNA

14. Transgenic Crops Engineered with Insecticidal Activity

15. Transgenic Crops Conferred with Herbicide Resistance

16. Growth Enhancement in Transgenic Fish

Part Four. Impact of Gene Cloning: Applications in Medicine and Related Areas

17. Microbial Production of Recombinant Human Insulin

18. Finding Disease-Causing Genes

19. Human Gene Therapy

20. Gene Targeting and Genome Editing

21. DNA Typing

22. Transpharmers: Bioreactors for Pharmaceutical Products

23. Animal Cloning

24. Whole Genome and Next Generation Sequencing

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