Epigenetics and Gene Expression in Cancer Inflammatory and Immune Diseases 1st Edition by Barbara Stefanska, David MacEwan 1493967414 9781493967414

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ISBN 10: 1493967414 

ISBN 13: 9781493967414

Author: Barbara Stefanska, David J. MacEwan 

This practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicology series, the book includes the kind of detail and implementation advice to encourage success in the lab.

Epigenetics and Gene Expression in Cancer Inflammatory and Immune Diseases 1st table of contents: 

1. High-Throughput Techniques for DNA Methylation Profiling
2. Reduced Representation Bisulfite Sequencing (RRBS) and Cell Sorting Prior to DNA Methylation Analysis in Psychiatric Disorders
3. Targeted DNA Methylation Analysis Methods
4. Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes
5. Synthesis and Application of Cell-Permeable Metabolites for Modulating Chromatin Modifications Regulated by α-Ketoglutarate-Dependent Enzymes
6. High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR
7. Methods for MicroRNA Profiling in Cancer
8. Microbiota and Epigenetic Regulation of Inflammatory Mediators
9. Optical Microscopy and Spectroscopy for Epigenetic Modifications in Single Living Cells
10. MicroRNAs in Therapy and Toxicity
11. Genetics and Epigenetics of Multiple Sclerosis

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Tags: Epigenetics, Gene Expression, Cancer Inflammatory, Immune Diseases, Barbara Stefanska, David MacEwan