Skin Stem Cells: Methods and Protocols Kursad Turksen

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This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents:

In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging

Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells

Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction

Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells

Isolation and Characterization of Cutaneous Epithelial Stem Cells
Stephanie R. Gillespie, David M. Owens
Pages 87-99
Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells

Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis

Isolation and Cultivation of Epidermal (Stem) Cells

One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen

Isolation and Cultivation of Skin-Derived Precursors

Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor

Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair

Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet

Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

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