Human Embryonic Stem Cell Protocols 3rd Edition Kursad 9781493926688

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Product details:

• ISBN 13: 9781493926688
• Author: Kursad Turksen

The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Table of contents:

Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts

Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel

Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix

Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate

Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells

Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR

Study of Gap Junctions in Human Embryonic Stem Cells

Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways

Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells

Genetic Manipulation of Human Embryonic Stem Cells

Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System

Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells

Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation

Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells

Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts

Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa

Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections

Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering

Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells

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