Open and Toroidal Electrophoresis Ultra High Separation Efficiencies in Capillaries Microchips and Slabs 1st Edition by Tarso Ledur Kist ISBN 9781119539247 1119539242

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Product details:
ISBN 10: 1119539242
ISBN 13: 9781119539247
Author: Tarso Ledur Kist
 

Open and Toroidal Electrophoresis Ultra High Separation Efficiencies in Capillaries Microchips and Slabs 1st Edition Table of contents:

1 Solvents and Buffer Solutions

1.1 Water as a Solvent

1.2 Binary Mixtures and Other Solvents

References

2 Fundamentals of Electrophoresis

2.1 Introduction

2.2 The Platforms

2.3 Electrophoresis

2.4 Electrophoresis of Single Molecules

2.5 Ionic Limiting Mobility

2.6 Bands, Fronts, Peaks, and Zones

2.7 The Isoelectric Point

2.8 Turbulent and Laminar Flow

2.9 Electroosmosis

2.10 Supression of EOF

2.11 Joule Effect and Heat Dissipation

2.12 Temperature Profiles

2.13 Molecular Diffusion and Band Broadening

2.14 Sample Stacking and Band Compression

2.15 Separation Modes

References

3 Open Layout

3.1 Introduction

3.2 Capillary Electrophoresis

3.3 Microchip Electrophoresis

3.4 Slab Electrophoresis

3.5 Performance Indicators for Open Layouts

References

4 Toroidal Layout

4.1 Introduction

4.2 Toroidal Capillary Electrophoresis

4.3 Toroidal Microchip Electrophoresis

4.4 Toroidal Slab Electrophoresis

4.5 Folding Geometries

4.6 Microholes and Connections

4.7 Reservoirs

4.8 Active and Passive Modes of Operation

4.9 Performance Indicators for Toroidal Layouts

References

5 Confronting Performance Indicators

5.1 Introduction

5.2 Performance Indicators from Experimental Data

5.3 Performance Indicators Predicted from Operational Parameters

References

6 High Voltage Modules and Distributors

6.1 Introduction

6.2 High Voltages in Open Layouts

6.3 High Voltages in Toroidal Layouts

References

7 Heat Removal and Temperature Control

7.1 Introduction

7.2 Temperature Gradients are Unavoidable

7.3 Temperature has Multiple Effects

7.4 Electrical Insulators with High Thermal Conductivity

7.5 Cooling Strategies Used in Capillary Electrophoresis

7.6 Cooling Strategies Used in Microchip Electrophoresis

7.7 Cooling Strategies Used in Slab Electrophoresis

7.8 Shear Rate of the Coolant

7.9 Final Considerations

References

Note

8 Detectors

8.1 Introduction

8.2 Fixed Point Detectors

8.3 Spatial Detectors (Scanners and Cameras)

8.4 Derivatization Reactions

References

9 Applications of Toroidal Electrophoresis

9.1 Introduction

References

Appendix A: Nomenclature

References

Appendix B: Species Concentration in Buffer Solutions

B.1 Acids (HnA)

B.2 Bases (B)

References

Appendix C: Electrophoresis

C.1 Free-Solution Electrophoretic Mobility

C.2 Mobility Dependence on Temperature

C.3 Transient Regimes

References

Appendix D: Electroosmosis

D.1 Slab and Microchips – Cartesian Coordinates

D.2 Capillaries – Cylindrical Coordinates

D.3 Zeta Potential

References

Appendix E: Molecular Diffusion

E.1 The Diffusion Equation

E.2 The Propagator

E.3 Application of Propagators to Bands at Rest

E.4 Application of Propagators to Bands in Movement

E.5 Bands and Peaks

References

Appendix F: Poiseuille Counter-flow

F.1 Introduction

F.2 Velocity Level Contours

F.3 Temperature Level Contours

F.4 Equalizing and

Reference

Appendix G: Cyclic On-column Band Compression

G.1 Introduction

G.2 Effect of Cyclic Band Compression Events on Variance

G.3 Number of Theoretical Plates

G.4 Number of Theoretical Plates per Unit Time

G.5 Height Equivalent of a Theoretical Plate

G.6 Resolution

G.7 Resolution per Unit Time

G.8 Band Capacity

G.9 Band Capacity per Unit Time

G.10 Detailed Calculation of and

References

Index

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