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Product details:
- ISBN-13 : 9781071604670
- Author: Thomas Proft
This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of contents:
Detection of Streptococcus pyogenes Virulence Factors
Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens
The emm-Cluster Typing System
Protocols for Tn-seq Analyses in the Group A Streptococcus
Genetic Manipulation of Group A Streptococcus—Gene Deletion by Allelic Replacement
Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging
Application of Group A Streptococcus Omics
Whole Genome Sequence Analysis and Population Genomics of Group A Streptococci
Group A Streptococcus Transcriptome Analysis
Protocol for Proteome Analysis of Group A Streptococcus
Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells
Assays to Investigate Group A Streptococcus Virulence Factors and Virulence Mechanisms
Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins
Expression and Purification of Collagen-Like Proteins of Group A Streptococcus
Detection of Fibronectin-Binding Proteins of Streptococcus pyogenes Using Ligand Blot Analysis
Morphology and Ultrastructure of Group A Streptococcus Biofilms
Dynamic Interactions of Group A Streptococcus with Host Macrophages
Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuoles
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