Group A Streptococcus Methods and Protocols 2020th Edition Thomas 9781071604670

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Product details:

• ISBN-13 ‏ : ‎ 9781071604670
• Author: Thomas Proft

This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents:

Detection of Streptococcus pyogenes Virulence Factors
Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens

The emm-Cluster Typing System

Protocols for Tn-seq Analyses in the Group A Streptococcus

Genetic Manipulation of Group A Streptococcus—Gene Deletion by Allelic Replacement

Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging

Application of Group A Streptococcus Omics

Whole Genome Sequence Analysis and Population Genomics of Group A Streptococci

Group A Streptococcus Transcriptome Analysis

Protocol for Proteome Analysis of Group A Streptococcus

Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

Assays to Investigate Group A Streptococcus Virulence Factors and Virulence Mechanisms

Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins

Expression and Purification of Collagen-Like Proteins of Group A Streptococcus

Detection of Fibronectin-Binding Proteins of Streptococcus pyogenes Using Ligand Blot Analysis

Morphology and Ultrastructure of Group A Streptococcus Biofilms

Dynamic Interactions of Group A Streptococcus with Host Macrophages

Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuoles

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