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Product details:
- ISBN-10 : 1071611577
- ISBN-13 : 978-1071611579
- Author: Haiming Cao
This detailed volume presents a comprehensive bioinformatic and experimental toolbox for prioritizing, annotating, and functionally analyzing long non-coding RNAs (lncRNAs). Playing a vital role in diverse biological progresses and human disease, lncRNAs have proven to be a challenging subject of study due to our limited understanding of their sequence-function relationships, lack of complete genetic annotation, and the unavailability of systems required to define their functional importance and molecular mechanisms, all of which this book seeks to address. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Functional Analysis of Long Non-Coding RNAs: Methods and Protocols provides atimely and convenient resource to facilitate the identification and characterization of disease-associated human lncRNAs, which aims to shed light on their role in biology and pathophysiology and ultimately lead toward novel therapeutic approaches targeting lncRNAs for the amelioration of human diseases.
Table of contents:
Bioinformatics Approaches for Functional Prediction of Long Noncoding RNAs
Visualization of lncRNA and mRNA Structure Models Within the Integrative Genomics Viewer
RNA Coding Potential Prediction Using Alignment-Free Logistic Regression Model
Classification of Long Noncoding RNAs by k-mer Content
Genome-Wide Computational Analysis and Validation of Potential Long Noncoding RNA-Mediated DNA–DNA–RNA Triplexes in the Human Genome
Using INFERNO to Infer the Molecular Mechanisms Underlying Noncoding Genetic Associations
A Bioinformatic Pipeline to Integrate GWAS and eQTL Datasets to Identify Disease Relevant Human Long Noncoding RNAs
AnnoLnc: A One-Stop Portal to Systematically Annotate Novel Human Long Noncoding RNAs
Annotation of Full-Length Long Noncoding RNAs with Capture Long-Read Sequencing (CLS)
Single-Cell Analysis of Long Noncoding RNAs (lncRNAs) in Mouse Brain Cells
Detection and Characterization of Ribosome-Associated Long Noncoding RNAs
Analysis of Annotated and Unannotated Long Noncoding RNAs from Exosome Subtypes Using Next-Generation RNA Sequencing
DMS-MaPseq for Genome-Wide or Targeted RNA Structure Probing In Vitro and In Vivo
Labeling and Purification of Temporally Expressed RNAs During the S-Phase of the Cell Cycle in Living Cells
A Quick Immuno-FISH Protocol for Detecting RNAs, Proteins, and Chromatin Modifications
M(R)apping RNA–Protein Interactions
In Vivo Administration of Therapeutic Antisense Oligonucleotides
CRISPR-Mediated Mutagenesis of Long Noncoding RNAs
In Vivo CRISPR/Cas9-Based Targeted Disruption and Knockin of a Long Noncoding RNA
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