Adeno-Associated Virus Vectors: Design and Delivery Michael 9781493991396

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Product details:

• ISBN 13: 9781493991396
• Author: Michael J. Castle

This volume provides a complete and timely guide to the use of adeno-associated virus (AAV)  vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Table of contents:

Design of AAV Vectors for Delivery of RNAi

Design of AAV Vectors for Delivery of Large or Multiple Transgenes

Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer

Quantitative and Digital Droplet-Based AAV Genome Titration

Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual DNA and AAV Vector Genomes

In Situ Hybridization for Detection of AAV-Mediated Gene Expression

Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina

AAV Delivery to the Central Nervous System

Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System

Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-transgenic Species

MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain

AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection

Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals

AAV Delivery to Sensory Systems

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