Advanced Methods in Structural Biology 1st Edition by Toshiya 9784431560302

Advanced Methods in Structural Biology 1st Edition by Toshiya – Ebook Instant Download/Delivery ISBN(s): 9784431560289,9784431560302,4431560289,4431560300

Product details:

• ISBN 13:  9784431560302
• Author: Toshiya

This book provides practical information on a whole set of protein experiments for advanced structural biology, such as X-ray crystallography, NMR, electron microscopy, advanced mass spectroscopy, and surface plasmon resonance, as well as a wide variety of expression systems including eukaryotic and in vitro expression. In the past decade, structural genomics studies have pushed forward the development of automated methods in the field of structural biology, however there is an increasing need for the structural analysis of difficult targets, such as large protein complexes and membrane proteins, which are hard to achieve using conventional automated methods, and require knowledge that goes beyond standard protein chemistry protocols. To address these problems and to help researchers develop novel methods, this volume provides examples of the development of new protein investigation methods and their theoretical background.This book particularly appeals to graduate students, postdoctoral researchers, young investigators wishing to gain a better understanding of the theory behind experiments, and those seeking further advanced, practical structural biology methods.

Table of contents:

Expression in Bacteria and Refolding

Expression of Proteins in Insect and Mammalian Cells

Application of MultiBac System to Large Complexes

Purification Using Affinity Tag Technology

Cell-Free Protein Production for Structural Biology

Purification and Crystallization of Membrane Proteins

Overview of Membrane Protein Purification and Crystallization

Cell-Free Synthesis of Membrane Proteins

Crystallization and Crystal Engineering

Screening of Cryoprotectants and the Multistep Soaking Method

Protein Modification for Crystallization

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