Cell Culture Bioprocess Engineering 2nd Edition by Wei Shou Hu ISBN 1498762859 978-1498762854

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ISBN 10: 1498762859
ISBN 13: 978-1498762854
Author: Wei Shou Hu

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized.

This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found 

Cell Culture Bioprocess Engineering 2nd Table of contents:

1 Overview of Cell Culture Processes

An Introduction to Cell Culture: A Historical Perspective

Cell Culture as Process Technology

Industrial Cell Lines

Other Production Systems

Manufacturing

Upstream Process

Product Quality in Cell Culture Processing

Quality of the Product

From Discovery to Clinical Products

2 Cell Biology for Bioprocessing

Introduction

Tissue Cells and Their Isolation

Cell Membrane

Cytoplasm and Organelles

Protein Secretion through the ER and Golgi Apparatus

Transport across Cellular Membranes

Cell Shape, Mechanics, and Movement

Growth Control

Concluding Remarks

3 Cell Physiology

Introduction

Glucose and Energy Metabolism

Regulation of Glucose Metabolism

Transport and Transporters

Metabolic Homeostasis and Lactate Consumption

Amino Acid Metabolism

Lipid Metabolism

Glycan Biosynthesis and Protein Glycosylation

Concluding Remarks

4 Product Quality and Process

Introduction

Quality of Protein Product

Structural Homogeneity of the Product

Biological Activities

Quality by Design (QbD) of Cell Culture Products

QTPP and CQAs

Quality Risk Management

Design Space and Control Strategy

QbD in Different Stages of the Product’s Life Cycle

Concluding Remarks

5 Stoichiometry and the Kinetics of Cell Cultivation

Introduction

Material Conversion in Cell Culture

Material Balance on Cell Growth

Kinetic Description of Cell Growth & Product Formation

Kinetic Model of Cell Growth

Modeling Productivity

Modeling a Cell Culture System

Concluding Remarks

6 Cell Line Development

Introduction

Transient Production vs. Stable Expression Cell Line

Host Cells

Generating a Recombinant Protein High-Producing Cell Line

Concluding Remarks: Genomic Technology & Cell Line Development

7 Medium Design for Cell Culture Processing

Introduction

Types of Media and Classes of Medium Components

Process Medium Design: Kinetics, Stoichiometry, Omics & Optimization

Components of Basal Medium

High Molecular Weight and Complex Supplements

Concluding Remarks

8 Cell Culture Bioreactors

Introduction

Basic Types of Bioreactors

Tissue Culture and Disposable Cell Culture Systems

Adherent Cell Support Systems

Bioreactors Developed for Cell Culture

Concluding Remarks

9 Fed-Batch Culture Processes

Introduction

Batch Cultures

Fed-Batch Cultures

Stoichiometric Feeding

Delivery of Feed Medium

Product Quality in Fed-Batch Culture

Concluding Remarks

10 Continuous Cell Culture Processes

Introduction

Analysis of Continuous Culture and Steady State

Methods of Cell Retention

Continuous Culture & Continuous Process

Concluding Remarks

11 Oxygen Transfer in Cell Culture Bioreactors

Introduction

Oxygen Concentration across the Gas–Liquid Interface

Oxygen Transfer at the Gas–Liquid Interface

Damage to Cells by Gas Sparging

Oxygen Transfer in Aggregates and Membrane Bioreactors

Concluding Remarks

12 Scaling Up and Scaling Down for Cell Culture Bioreactors

Introduction

Mechanical Agitation

Power Consumption for Mixing Tanks

Mixing Time

Scaling Up and Mechanical Forces on Cells

Oxygen and Carbon Dioxide Transfer

Process Scale-Up and Scale-Down Models

Concluding Remarks

Appendix A: Cell Culture Process Data Analysis

Overview of Process Data

Data Processing Pipeline

Metabolic Flux Analysis

Time Series Data Analysis

Aggregation and Analysis of Process Data

Concluding Remarks

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Tags: Wei Shou Hu, Cell Culture, Bioprocess Engineering