Developmental Biology of the Sea Urchin and Other Marine Invertebrates Methods and Protocol 2nd Edition by David Carroll, Stephen Stricker 1627039734 978-1627039734

Developmental Biology of the Sea Urchin and Other Marine Invertebrates Methods and Protocol 2nd Edition by David J. Carroll, Stephen A. Stricker – Ebook PDF Instant Download/DeliveryISBN:  1627039734, 978-1627039734 

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Product details:

ISBN-10 ‏ : ‎ 1627039734

ISBN-13 ‏ : ‎ 978-1627039734

Author: David J. Carroll, Stephen A. Stricker

In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Developmental Biology of the Sea Urchin and Other Marine Invertebrates Methods and Protocol 2nd table of contents:

1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)
2. Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata
3. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae
4. Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis
5. Staining and Tracking Methods for Studying Sponge Cell Dynamics
6. Microscopy Studies of Placozoans
7. Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata
8. Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging
9. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization
10. Quantifying Cell Proliferation During Regeneration of Aquatic Worms
11. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis
12. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond
13. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation
14. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis
15. RNA Interference on Regenerating Holothurian Gut Tissues
16. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos
17. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase
18. Functional Studies of Trichoplax adhaerens Voltage-Gated Calcium Channel Activity
19. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna

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Tags: Developmental Biology, the Sea Urchin, Other Marine, Invertebrates, Protocol, David Carroll, Stephen Stricker