Electrophoresis in practice a guide to methods and applications of DNA and protein separations 5th Edition by Reiner Westermeier ISBN 9783527695195 3527695192

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Product details:

ISBN 10: 3527695192

ISBN 13: 9783527695195

Author: Reiner Westermeier 

 This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis.
The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.

Table of contents: 

Part I: Fundamentals

1. Electrophoresis

2. Isotachophoresis

3. Isoelectric Focusing

4. High-Resolution Two-Dimensional Electrophoresis

5. Protein Sample Preparation

6. Protein Detection

7. Blotting

Part II: Equipment and Methods

8. Special Laboratory Equipment

9. Consumables

10. Chemicals

Method 1: Page Of Dyes

Method 2: Agarose And Immunoelectrophoresis

Method 3: Titration Curve Analysis

Method 4: Native PAGE In Amphoteric-Buffers

Method 5: Agarose IEF

Method 6: PAGIEF In Rehydrated Gels

Method 7: Horizontal SDS-PAGE

Method 8: Vertical PAGE

Method 9: Semidry Blotting Of Proteins

Method 10: IEF In Immobilized pH Gradients

Method 11: High-Resolution 2D Electrophoresis

Method 12: PAGE Of DNA Fragments

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Tags: Reiner Westermeier, Electrophoresis, Practice, DNA Separation, Protein Separation, Methods, Applications