Electrophoretic Separation of Proteins: Methods and Protocols Biji T. Kurien

This completed downloadable of Electrophoretic Separation of Proteins: Methods and Protocols Biji T. Kurien 

Instant downloaded Electrophoretic Separation of Proteins: Methods and Protocols Biji T. Kurien pdf docx epub after payment.

Product details:

• ISBN-10 ‏ : ‎ 1493987925
• ISBN-13 ‏ : ‎ 978-1493987924
• Author:  Biji T. Kurien

This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.

Table of contents:

How It All Began: A Personal History of Gel-Electrophoresis.

– Introduction to Protein Electrophoresis.

– Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilisation, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation.

– Lysis Buffer Choices are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis.

– The Cydex Blue Assay: A One Step Protein Assay for Samples Prior to SDS Electrophoresis.

– Cellulose-Acetate Electrophoresis of Hemoglobin.

– Native Polyacrylamide Gels.

– Isoelectric Focusing on Non-Denaturing Gels.

People also search:

how does electrophoresis separate molecules

how are proteins separated in gel electrophoresis

how does electrophoresis separate dna fragments

how electrophoresis works