Fundamentals of MALDI ToF MS Analysis Applications in Bio diagnosis Tissue Engineering and Drug Delivery 1st Edition by Samira Hosseini,Sergio Martinez 9789811023569 9811023565

1 Principles and Mechanism of MALDI-ToF-MS Analysis

Abstract

1.1 Background of MALDI-ToF-MS

1.2 Applications of MALDI-ToF-MS

1.2.1 Biochemistry

1.2.2 Organic Chemistry

1.2.3 Polymer Chemistry

1.2.4 Microbiology

1.2.5 Medicine

1.2.6 Nanotechnology

1.3 Ionization Mechanism

1.4 Time of Flight Mass Spectrometry Mechanism

1.5 Detection Modes

1.5.1 Linear Mode

1.5.2 Reflection Mode

1.6 Laser Range in MALDI

1.7 Matrix Selection

1.7.1 Limitations of Matrices

1.8 Ionizing Agent Selection

1.9 Fundamentals of Sample Preparation for MALDI Analysis

1.9.1 Analyzable and Non-analyzable Polymers

1.9.2 Sample Preparation Techniques

1.9.2.1 Dried-Droplet

1.9.2.2 Fast Crystallization

1.9.2.3 Spin Coating

1.9.2.4 Layer by Layer

1.9.2.5 Electrospray Deposition

1.10 Mass Discrimination

1.10.1 Alternative Solutions for Overcoming Mass Discrimination

1.11 MALDI Coupling

1.11.1 Ion-Mobility Time of Flight Mass Spectrometry (IMS-ToF-MS)

1.11.2 Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS)

1.11.3 Thin Layer Chromatography (TLC)

1.11.4 Size Exclusion Chromatograph (SEC)

References

2 Fundamentals of Biosensors and Application of MALDI-ToF-MS in Bio-diagnostic Domain

Abstract

2.1 Biosensors

2.2 Synthesis of Poly Methyl Methacrylate-Co-Methacrylic Acid P(MMA-Co-MAA) and Preparation of the B

2.3 Sample Preparation for MALDI Analysis

2.4 MALDI Data Interpretation

2.4.1 Different Species as the Possible Products of Soft Ionization

2.4.2 General Structure of P(MMA-Co-MAA)

2.4.3 MALDI Analysis of PMMA

2.4.4 MALDI Analysis of P(MMA-Co-MAA)

2.4.5 End-Group Analysis

2.5 Role of MALDI Analysis in Bio-sensing Application of P(MMA-Co-MAA)

2.5.1 Reaction’s Parameters

2.5.2 Chemistry Aspect

2.5.3 Physical Aspect

References

3 Fundamentals of Tissue Engineering and Application of MALDI-ToF-MS in Analysis of the Scaffold Mat

Abstract

3.1 Tissue Engineering

3.2 Synthesis of Poly Octanediol Citrate Acid-Sebacate Acid P(OCS) and Preparation of the Scaffold

3.3 MALDI Analysis of P(OCS) and Sample Preparation

3.4 MALDI Data Interpretation

3.5 Role of MALDI Analysis in Tissue Engineering Application of P(OCS)

3.5.1 Reaction’s Parameters

3.5.2 Chemistry Aspect

3.5.3 Mechanical Aspect

References

4 Fundamentals of Drug Delivery Systems and Application of MALDI-ToF-MS in Drug Delivery

Abstract

4.1 Drug Delivery

4.2 Synthesis of PCLT-CA and Preparation of Elastomeric Films and Coatings

4.3 Sample Preparation for MALDI Analysis

4.4 MALDI Data Interpretation

4.5 The Role of MALDI Analysis in Drug Delivery Application of PCLT-CA

4.5.1 Chemistry Aspect

4.5.2 Mechanical Aspect