Handbook of Surface Plasmon Resonance 2nd Edition by Richard Schasfoort ISBN 1788011392 9781788011396

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ISBN 10: 1788011392

ISBN 13: 978-1788011396

Author:  Richard B. M. Schasfoort 

Handbook of Surface Plasmon Resonance 2nd Edition: 

Handbook of Surface Plasmon Resonance 2nd Edition Table of contents:

Chapter 1: Introduction to Surface Plasmon Resonance
1.1 Introduction to Surface Plasmon Resonance
1.2 What is a Biosensor?
    1.2.1 A Simple Experiment
    1.2.2 From Dip to Real-time Measurement
1.3 How to Construct an SPR Assay
    1.3.1 The Steps of an Assay
    1.3.2 Concentration Determination
    1.3.3 Determination of Kinetic Parameters
    1.3.4 Basics of Instrumentation
1.4 Kinetics of Biomolecular Interactions
    1.4.1 Mass Transport-controlled Kinetics
    1.4.2 Calibration-Free Concentration Analysis (CFCA)
    1.4.3 Interaction-controlled Kinetics
    1.4.4 Equilibrium Analysis
1.5 Buffer Solutions for Measuring the Analysis Cycle
    1.5.1 Baseline or System Buffer
    1.5.2 Regeneration Buffer
1.6 SPR-based Immunoassays
    1.6.1 Direct Assay
    1.6.2 Competition Assay
    1.6.3 Inhibition Assay
    1.6.4 Sandwich Assay with Secondary Antibody and Signal Enhancers
1.7 How to Read This Book
1.8 Questions
References

Chapter 2: History and Physics of Surface Plasmon Resonance
2.1 Introduction
2.2 History
    2.2.1 Early History of SPR Biosensors
    2.2.2 History of SPR Biosensors After 1990
2.3 Surface Plasmon Theory
    2.3.1 The Evanescent Wave
    2.3.2 Surface Plasmon Dispersion Equations; Resonance
    2.3.3 Excitation of Surface Plasmons
    2.3.4 Surface Plasmon Properties
    2.3.5 Choice of Experimental Parameters
    2.3.6 Optimizing SPR Imaging Performance
2.4 SPR Instrument Optics
    2.4.1 Fixed Angle
    2.4.2 Fan-shaped Beam
    2.4.3 Scanning Angle
    2.4.4 Grating Coupler
    2.4.5 Fiber-based SPR Sensors
    2.4.6 Other Optical Systems
    2.4.7 SPR Imaging Instruments
2.5 Concluding Remarks
2.6 Questions
References

Chapter 3: Surface Plasmon Resonance Instruments
3.1 Introduction
3.2 The Cornerstones of SPR Technology
3.3 General Optical Requirements for SPR Instruments
3.4 SPR Liquid Handling Systems
    3.4.1 Cuvette Systems
    3.4.2 Flow Systems
3.5 SPR Instruments: State of the Art
    3.5.1 Examples of Fan-shaped Beam SPR Instruments
    3.5.2 Examples of Fixed- and Scanning-angle SPR Instruments
    3.5.3 Examples of Other Label-free Biosensing Instruments
    3.5.4 Examples of SPR Imaging Instruments
3.6 Biacore SPR Systems of GE Healthcare
    3.6.1 Biacore T100, T200, and S200
    3.6.2 Biacore A100/4000
    3.6.3 Biacore 8K
3.7 Conclusions
3.8 Questions
References

Chapter 4: SPRpages – Getting a Feeling for the Curves
4.1 Introduction
4.2 The Exponential
4.3 A Curve
    4.3.1 Baseline
    4.3.2 Association
    4.3.3 Steady State
    4.3.4 Dissociation
    4.3.5 Response Units
    4.3.6 Equilibrium and Saturation
    4.3.7 Rmax Value
    4.3.8 Curve Response
    4.3.9 Req value
    4.3.10 Time to Reach Equilibrium
4.4 Curve Examples
    4.4.1 Curve Shape
    4.4.2 Exponential Curve
    4.4.3 Mass Transport Limitation (MTL)
    4.4.4 Biphasic Curves
    4.4.5 Drift, Jumps and Spikes
4.5 Experimental Setup
    4.5.1 Analyte Concentration Range
    4.5.2 Blank Injections
    4.5.3 Multi-cycle Kinetics
    4.5.4 Single-cycle Kinetics
    4.5.5 Equilibrium Analysis
    4.5.6 Fast Kinetics
    4.5.7 Decaying Surface
4.6 Sensorgram Quality
4.7 The Affinity Plot; Getting a Feeling for the Numbers
4.8 Curve Fitting
    4.8.1 Residual Plot
    4.8.2 Local and Global Fitting
    4.8.3 Deviations from a 1 : 1 Interaction
4.9 Interaction Validation
4.10 Publications
    4.10.1 Evaluating Published Results
    4.10.2 Minimal Requirements for Describing a Biosensor Experiment
4.11 SPR Simulation
    4.11.1 Different Analyte Concentration (Same Kinetics)
    4.11.2 Different Association Rate (Different Affinity)
    4.11.3 Different Dissociation Rate (Different Affinity)
    4.11.4 Different Kinetics (Same Affinity)
    4.11.5 Different Injection Times (Same Kinetics)
    4.11.6 Different Dissociation Times (Same Kinetics)
4.12 Questions
4.13 Glossary
References

 

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