Hepatitis C Virus Protocols 1st edition by Mansun Law 1493989751 9781493989751

Hepatitis C Virus Protocols 1st edition by Mansun Law – Ebook PDF Instant Download/DeliveryISBN:  1493989751, 9781493989751 

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Product details:

ISBN-10 :  1493989751 

ISBN-13 :  9781493989751

Author:  Mansun Law 

This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c virus(HCV) cloning, culture, and purification, HCV life cycle, host immune responses, and small animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Hepatitis C Virus Protocols aims to ensure successful results in the further study of this vital field.

Hepatitis C Virus Protocols 1st Table of contents:

1. In Situ Detection of HCV
2. In Situ Detection of Hepatitis C Viral Antigens
3. In Situ Hybridization and the Detection and Localization of HCV RNA
4. The In Situ Detection of PCR-Amplified Hepatitis C RNA
5. lmmunoelectron Microscopic Characterization of HCV
6. Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones
7. Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing
8. Use of a Discistronic Vector for the Quantitation of HCV IRES Activity
9. Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli
10. Expression and Characterization of the HCV NS2 Protease
11. Expression and Characterization of HCV NS3 Protease
12. Expression and Characterization of the HCV NS3 Helicase Domain
13. Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)
14. Detection and Molecular Cloning of the Extreme 3′-End of HCV
15. In Vitro Stability of Hepatitis C Virus RNA
16. Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
17. HCV-Specific Immunologic Response
18. Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC
19. Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay

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