High Throughput Protein Production and Purification Methods and Protocols 1st edition by Renaud Vincentelli 1493996231 ‎ 9781493996230

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ISBN 10:1493996231 
ISBN 13:9781493996230 
Author:Renaud Vincentelli 
This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.

High Throughput Protein Production and Purification Methods and Protocols 1st Table of contents: 

General Procedures for High-Throughput Protein Production

Overview of High-Throughput Cloning Methods for the Post-genomic Era

Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins

Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins

High-Throughput Protein Production in Yeast

A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells

A High-Throughput Automated Protein Folding System

High-Throughput Protocols Adapted to the Production of Specific Protein Families

High-Throughput Production of Oxidized Animal Toxins in Escherichia coli

High-Throughput Purification of Protein Kinases from Escherichia coli and Insect Cells

Parallelized Microscale Expression of Soluble scFv

High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

High-Throughput Protein Production of Membrane Proteins in Saccharomyces cerevisiae

High-Throughput E. coli Cell-Free Expression: From PCR Product Design to Functional Validation of GPCR

High-Throughput Site-Directed Mutagenesis

High-Throughput Protein Production Combined with High Throughput Micro-Characterization for Protein Stability, Solubility, Quality and Interactions

Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants

High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System

High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization

 

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Renaud Vincentelli,Purification,Production,Throughput