Lipidomics Methods and Protocols 1st Edition Sanjoy 1493969951 9781493969951

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Product details:

• ISBN-10 ‏ : ‎ 1493969951
• ISBN-13 ‏ : ‎ 9781493969951
• Author: Sanjoy K. Bhattacharya

This volume explores lipidomics through protocols that focus on areas of utility, techniques, and bioinformatics advancements. The protocols in this book cover topics such as isolation of specific membranes and specialized fractionation of subcellular compartments, and computational and functional analysis of lipid metabolizing enzymes. Each protocol also discusses methods of dealing with fractionated organelles―cells to whole organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents:

1 Lipid Sample Preparation for Biomedical Research

2 Lipid Extraction Techniques for Stable Isotope Analysisand Ecological Assays

3 Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells

4 Isolation of Neuronal Synaptic Membranes by SucroseGradient Centrifugation

5 Sample Preparation and Analysis for Imaging Mass Spectrometry

6 Direct Measurement of Free and Esterified Cholesterol Massin Differentiated Human Podocytes: A TLC and Enzymatic Assay-Based Method

7 High-Performance Chromatographic Separation of Cerebrosides

8 Lipid Identification by Untargeted Tandem Mass SpectrometryCoupled with Ultra-High-Pressure Liquid Chromatography

9 Utility of Moderate and High-Resolution Mass Spectrometryfor Class-Specific Lipid Identification and Quantification

10 A Robust Lipidomics Workflow for Mammalian Cells, Plasma,and Tissue Using Liquid-Chromatography High-Resolution TandemMass Spectrometry

11 Combined Use of MALDI-TOF Mass Spectrometry

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