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Product details:
- ISBN-10: 1118969553
- ISBN-13: 978-1118969557
- Author:
PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS―EMPHASIZING MASS SPECTROMETRY THROUGHOUT
Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments.
The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures.
Table of contents:
1 Protein Analysis by Shotgun Proteomics1
2 Quantitative Proteomics for Analyses of Multiple Samples in Parallel with Chemical Perturbation
3 Chemoproteomic Analyses by Activity‐Based Protein Profiling
4 Activity‐Based Probes for Profiling Protein Activities
5 Chemical Probes for Proteins and Networks
6 Probing Biological Activities with Peptide and Peptidomimetic Biosensors
7 Chemoselective Tagging to Promote Natural Product Discovery
8 Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics
9 Tracing Endocytosis by Mass Spectrometry
10 Functional Identification of Target by Expression Proteomics (FITExP)
11 Target Discovery Using Thermal Proteome Profiling
12 Chemical Strategies to Glycoprotein Analysis
13 Proteomic Analysis of Protein–Lipid Modifications: Significance and Application
14 Site‐Specific Characterization of Asp‐ and Glu‐ADP‐Ribosylation by Quantitative Mass Spectrometry
15 MS‐Based Hydroxyl Radical Footprinting: Methodology and Application of Fast Photochemical Oxidation of Proteins (FPOP)
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