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Product details:
- ISBN-10 : 1493933671
- ISBN-13 : 9781493933679
- Author: Francis Martin
This volume seeks to understand how organisms and gene functions are influenced by environmental cues while accounting for variation that takes place within and among environmental populations and communities. Microbial Environmental Genomics (MEG) guides readers through methods to analyse the diversity of different organism types (archaea, bacteria, fungi, protists and microfauna), interactions between fungi and trees, and methods to identify and characterize functions and functional diversity of both pro- and eukaryotes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of contents:
Contents: “Deciphering archaeal communities” omics tools in the study of archaeal communities / Lejla Pašić , Ana-Belen Martin-Cuadrado, and Purificación López-García — Investigating the endobacteria which thrive in arbuscular mycorrhizal fungi / Alessandro Desirò, Alessandra Salvioli, and Paola Bonfante — GenoSol platform : a logistic and technical platform for conserving and exploring soil microbial diversity / Samuel Dequiedt, Pierre-Alain Maron, and Lionel Ranjard — Sample preparation for fungal community analysis by high-throughput sequencing of barcode amplicons / Karina Engelbrecht Clemmensen … [et al.] — Fungal communities in soils : soil organic matter degradation / Tomáš Větrovský, Martina Štursová, and Petr Baldrian — DNA-based characterization and identification of arbuscular mycorrhizal fungi species / Carolina Senés-Guerrero and Arthur Schüssler — Molecular identification of soil eukaryotes and focused approaches targeting protist and faunal groups using high-throughput metabarcoding / G. Arjen de Groot, Ivo Laros, and Stefan Geisen — Identification and in situ distribution of a fungal gene marker : the mating type genes of the black truffle / Herminia De la Varga and Claude Murat — Stable-isotope probing RNA to study plant/fungus interactions / Amandine Lê Van … [et al.] — Targeted gene capture by hybridization to illuminate ecosystem functioning / Céline Ribière … [et al.] — Hybridization of environmental microbial community nucleic acids by GeoChip / Joy D. Van Nostrand … [et al.] — Reconstruction of transformation processes catalyzed by the soil microbiome using metagenomic approaches / Anne Schöler … [et al.] — MG-RAST, a metagenomics service for analysis of microbial community structure and function / Kevin P. Keegan, Elizabeth M. Glass, and Folker Meyer — Analysis of active methylotrophic communities : when DNA-SIP meets high-throughput technologies / Martin Taubert … [et al.] — Functional metagenomics : construction and high-throughput screening of fosmid libraries for discovery of novel carbohydrate-active enzymes / Lisa Ufarté … [et al.] — Metatranscriptomics of soil eukaryotic communities / Rajiv K. Yadav … [et al.] — Analysis of ancient DNA in microbial ecology / Olivier Gorgé … [et al.].
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