Micropropagation of orchids 3rd Edition by Joseph Arditti, Tim Wing Yam 1119187059 9781119187059

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Product details:

ISBN 10:1119187059 

ISBN 13: 9781119187059

Author: Joseph Arditti, Tim Wing Yam 

Divided into three volumes, Micropropagation of Orchids Third Edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new and/or revised information about:

Culture media and vessels
Techniques and procedures for both orchids which were previously cultured and for those which were not
Plant hormones and growth regulators
Media components
Methods for tissue decontamination
Historical information
Procedures for the cultivation for plantlets which have been removed from flasks
Sources of light and illumination methods
Written by two globally acknowledged experts in the field, the third edition of this definitive text on the micropropagation of orchids is a detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro and is intended for researchers in plant science and propagation, professional and amateur orchid growers, and plant breeding professionals. Much of the general information about techniques and procedures can be applied to plants other than orchids.

 

Micropropagation of orchids 3rd Table of contents: 

Chapter 1: History

Terminology

Origins of Orchid Micropropagation

Plant Hormones and Propagation Additives of Plant Origin

Culture of Tissues and Organs

Early Tissue Culture Attempts

Culture of Stem Tips

First Micropropagation of Orchids

The Second Aseptic Culture of an Orchid Explant

Plant Diseases and Meristems

The Third Aseptic Culture of an Orchid Explant

The Fourth Aseptic Culture of an Orchid Explant

Who Pioneered Micropropagation?

Root Cultures

Leaf Cultures

Stems

Flower Buds, Flowers, Floral Segments, and Reproductive Organs

Inflorescences

A Patent

Doubtful Claims

Mutations

Theft in Vitro

Darkening of Culture Media

Anticontaminants

Cell and Protoplast Culture

Flowering in Vitro,  

The Future

Chapter 2: General Outline of Techniques and Procedures

Media Components

Macroelements

Microelements

Iron

Auxins and Anti-auxin

Chitosan

Cytokinins

Gibberellins and Antigibberellin

Abscisic Acid

Ethylene and Ethylene Inhibitor

Amino Acids

Polyol

Polyamines

Phloroglucinol

Jasmonates

Vitamins

Nucleotides and Nucleic Acids

Organic Acids

Banana

Coconut Water

Hydrolysates and Autolysates

Sugars

Anticontaminants

Charcoal

Solvents

Solidifiers

pH

Recipes in this Book

Stock Solutions

State of the Medium

Sterilization

Autoclaves

Filtration

Sterilization in Microwave Ovens

Solvents

Open Flame

Liquids

Surface Decontamination

Gas Sterilization

Preparation of the Medium

Culture Vessels

Culture Conditions

Temperature

Agitation

Illumination

Carbon Dioxide

Placing Plant Material in Culture

Seeds

Tissue Explants

Internal Contaminants

Enzymes for Protoplast Isolation

Work Area

Laminar-flow Hoods

Sterile Rooms

Sterile Boxes

Clean Laboratory Bench

Washing Glassware

Chapter 3: Methods for Specific Genera

Acampe

Aeridachnis

Aerides

Aeridovanda

Anacamptis

Angraecum

Anoectochilus

Ansellia

Arachnis

Arachnostylis

Aranda

Aranthera

Arundina

Ascocenda

Ascocentrum

Ascofinetia

Barlia

Bletilla

Brassavola

Brassia

Brassocattleya

 

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Tags: Micropropagation, orchids, Joseph Arditti, Tim Wing Yam