Mitochondrial Gene Expression Methods and Protocols 1st edition by Michal Minczuk, Joanna Rorbach ISBN 1071608339 9781071608333

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ISBN 10:     1071608339
ISBN 13: 978-1071608333
Author:      Michal Minczuk, Joanna Rorbach

Mitochondrial Gene Expression Methods and Protocols 1st Table of contents:

1. In vitro Analysis of mtDNA Replication – This technique focuses on the study of mitochondrial DNA replication outside of living cells, typically in a controlled laboratory environment using purified proteins or cell extracts.

2. In vivo Single Molecule Analysis of mtDNA Replication – High-resolution imaging to study mtDNA replication directly within living cells at the single-molecule level.

3. In Vitro Reconstitution of Human Mitochondrial Transcription – A method for reconstructing mitochondrial transcription processes in vitro to better understand how mitochondrial genes are transcribed.

4. Circular RNA Sequencing of Mitochondrial Transcriptomes – A specialized sequencing technique to study circular RNAs in the mitochondria, which may play a role in regulating mitochondrial function.

5. Detection of 5-formylcytosine in Mitochondrial Transcriptomes – A method to identify specific modifications to mitochondrial RNA that may impact its stability or translation.

6. Visualization of Mitochondrial RNA Granules – Using 5-bromouridine labeling to visualize mitochondrial RNA granules in cultured cells, helping to study RNA processing and transport.

7. Quantitative Proteomics with Stable Isotope Labeling – A technique used to measure the abundance and modifications of proteins in Drosophila (fruit flies), which can be applied to mitochondrial studies.

8. Mito-Ψ-Seq for Mapping Pseudouridine in Mitochondrial RNA – High-throughput sequencing method used to identify pseudouridine modifications in mitochondrial RNA, a key modification impacting RNA stability and translation.

9. High-throughput Detection of mtDNA Mutations – Methods to identify mutations in mtDNA that lead to errors in tRNA processing, contributing to mitochondrial diseases.

10. Analysis of Mitochondrial RNA Turnover – Techniques to measure how quickly mitochondrial RNA is degraded in human cells, providing insight into RNA stability and turnover.

11. RNA Crosslinking to Study Mitochondrial RNA-binding Proteins – A method to identify proteins that interact with mitochondrial RNA, crucial for understanding RNA regulation.

12. Visualization of Mitochondrial Protein Synthesis – Techniques to observe mitochondrial ribosomal RNA and the synthesis of proteins in human cells.

13. Mitoribosome Profiling and Cryo-EM – Advanced imaging and profiling methods to study mitochondrial ribosomes and translation processes, including cryo-electron microscopy for high-resolution imaging of these structures.

14. Blue Native Electrophoresis for OXPHOS Complexes – A technique to study mitochondrial oxidative phosphorylation (OXPHOS) complexes, crucial for energy production in cells.

15. Identification of Mitochondrial Protease Substrates – Methods to identify proteins that are substrates for mitochondrial proteases, shedding light on mitochondrial protein degradation pathways.

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