Mucins Methods and Protocols Methods in Molecular Biology 2763 1st Edition Kameyama 1071636693 9781071636695

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Product details:

• ISBN-10 ‏ : ‎ 1071636693
• ISBN-13 ‏ : ‎ 9781071636695
• Author: Kameyama

This volume explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents:

Part I: Extraction and Separation
Chapter 1: Preparation of Jellyfish Mucin
Chapter 2: Extraction of Mucin from Rodent Feces and Determination of O-Linked Oligosaccharide
Chapter 3: Preparation of Soluble Mucin Solutions from the Salivary Glands
Chapter 4: Isolation of Membrane Bound Mucins from Human Bronchial Epithelial Cells
Chapter 5: Extraction and Fractionation of Human Gastric Mucins from Gastric Juice
Chapter 6: Extraction of Mucins from the Mammalian Intestinal Tract
Chapter 7: Supported Molecular Matrix Electrophoresis
Part II: Staining, Detection, and Quantitation
Chapter 8: Supported Immunohistochemical Staining of Mucins
Chapter 9: Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membrane
Chapter 10: Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane
Chapter 11: Quantitation of MUC5AC and MUC5B by Stable Isotope Labeling Mass Spectrometry
Part III: Preparation and Analysis of Mucin Glycans
Chapter 12: Preparation of O-Glycans from Mucins Using Hydrazine Treatment
Chapter 13: Eliminative Oximation of O-Glycans from Mucins
Chapter 14: 9-Fluorenylmethyl Chloroformate Labeling for O-Glycan Analysis
Chapter 15: Liquid Chromatography and Capillary Electrophoresis Analysis of 2AA-Labeled O-Glycans
Chapter 16: Preparation of Mucin Glycopeptides by Organic Synthesis
Chapter 17: MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols Released from Mucins
Chapter 18: Structural Elucidation of Sialylated O-Glycan Alditols Obtained from Mucins by Mass
Chapter 19: Differential Glycoform Analysis of MUC1 Derived from Biological Specimens Using an
Chapter 20: ISOGlyP: O-Glycosylation Site Prediction Using Peptide Sequences and GALNTs

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