Nanotoxicity Methods and Protocols 1st Edition by Qunwei Zhang ISBN 1493989154 978-1493989157

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ISBN 10: 1493989154 

ISBN 13: 978-1493989157

Author: Qunwei Zhang

 This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field.

Table of contents: 

1. Historical Overview of Nanotechnology and Nanotoxicology

2. Characterization of Nanomaterials for Toxicological Studies

3. Methods for Understanding the Interaction Between Nanoparticles and Cells

4. Single-Cell Gel Electrophoresis (Comet) Assay in Nano-genotoxicology

5. Single-Cell Nanotoxicity Assays of Superparamagnetic Iron Oxide Nanoparticles

6. Western Blot Analysis

7. Application of Reverse Transcription-PCR and Real-Time PCR in Nanotoxicity Research

8. Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase Activity Indirectly

9. Enzyme-Linked Immunosorbent Assay of IL-8 Production in Response to Silver Nanoparticles

10. Metabolomics Techniques in Nanotoxicology Studies

11. Nanoparticle Uptake Measured by Flow Cytometry

12. Determining Biological Activity of Nanoparticles as Measured by Flow Cytometry

13. Whole Cell Impedance Biosensoring Devices

14. Free Energy Calculation of Permeant–Membrane Interactions Using Molecular Dynamics Simulations

15. Screening of Fullerene Toxicity by Hemolysis Assay

16. Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model

17. In Vivo Methods of Nanotoxicology

18. The Luminescent Bacteria Test to Determine the Acute Toxicity of Nanoparticle Suspensions

19. The Primacy of Physicochemical Characterization of Nanomaterials for Reliable Toxicity Assessment: A Review of the Zebrafish Nanotoxicology Model

20. Application of Embryonic and Adult Zebrafish for Nanotoxicity Assessment

21. Applications of Subsurface Microscopy

22. Application of ICP-MS for the Study of Disposition and Toxicity of Metal-Based Nanomaterials

23. Quantitative Nanoparticle Organ Disposition by Gel Permeation Chromatography

24. Physiologically Based Pharmacokinetic Modeling for Nanoparticle Toxicity Study

25. Biophysical Methods for Assessing Plant Responses to Nanoparticle Exposure

26. In Vivo Nanotoxicity Assays in Plant Models

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Tags: Qunwei Zhang, Nanotoxicity, Methods, Protocols