Oral Biology Molecular Techniques and Applications 2nd Edition Gregory 9781493966851

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Product details:

• ISBN 13: 9781493966851
• Author: Gregory J. Seymour

This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents:

Salivary Diagnostics Using Purified Nucleic Acids

RNA Sequencing Analysis of Salivary Extracellular RNA

Qualitative and Quantitative Proteome Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry

Antioxidant Micronutrients and Oxidative Stress Biomarkers

NMR-Based Metabolomics of Oral Biofluids

Gene Therapy of Salivary Diseases

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