Protein ligand interactions methods and applications 3rd Edition by Tina Daviter, Christopher M Johnson, Stephen H McLaughlin, Mark A Williams ISBN 1071611968 978-1071611968

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Product details:

ISBN 10: 1071611968 

ISBN 13: 978-1071611968

Author:  Tina Daviter, Christopher M. Johnson, Stephen H. McLaughlin, Mark A. Williams

This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.

Table of contents: 

1. Assessing and Improving Protein Sample Quality

2. A Familiar Protein–Ligand Interaction Revisited with Multiple Methods

3. Interactions of a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow Kinetics

4. Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins

5. Isothermal Titration Calorimetry

6. Measuring the KD of Protein–Ligand Interactions Using Microscale Thermophoresis

7. Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D): Preparing Functionalized Lipid Layers for the Study of Complex Protein–Ligand Interactions

8. Indirect Detection of Ligand Binding by Thermal Melt Analysis

9. The Use of Acoustic Mist Ionization Mass Spectrometry for High-Throughput Screening

10. Ligand Discovery: High-Throughput Binding: Fluorescence Polarization (Anisotropy)

11. Fragment Screening by NMR

12. A Quick Primer in Fluorescence-Based Equilibrium and Pre-steady State Methods for Determining Protein–Nucleotide Affinities

13. Measurement of Nucleotide Hydrolysis Using Fluorescent Biosensors for Phosphate

14. Gel-Based Analysis of Protein–Nucleic Acid Interactions

15. Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome Using switchSENSE

16. Biolayer Interferometry: Protein–RNA Interactions

17. Analysis of Protein–DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip

18. Characterization of Protein–Nucleic Acid Complexes by Size-Exclusion Chromatography Coupled with Light Scattering, Absorbance, and Refractive Index Detectors

19. Analytical Ultracentrifugation for Analysis of Protein–Nucleic Acid Interactions

20. Studying RNA–Protein Complexes Using X-Ray Crystallography

21. Flow Linear Dichroism of Protein–Membrane Systems

22. Probing Protein–Membrane Interactions and Dynamics Using Hydrogen–Deuterium Exchange Mass Spectrometry (HDX-MS)

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Tags: Tina Daviter, Christopher M Johnson, Stephen H McLaughlin, Mark A Williams, Protein, Ligand, Interactions, Methods, Applications