Proteus mirabilis Methods and Protocols 1st edition by Melanie M. Pearson ISBN 1493996001 9781493996001

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ISBN 10:      1493996001
ISBN 13: 978-1493996001
Author:      Melanie M. Pearson

Proteus mirabilis: Methods and Protocols 1st Table of contents:

Part I: Culturing and Growth of Proteus mirabilis

1. Isolation and Culturing of Proteus mirabilis

   • Selective Media for Isolation
   • Growth Conditions for Optimal Cultures
   • Techniques for Storing Cultures

2. Identification and Characterization of Proteus mirabilis

   • Phenotypic Characterization (Morphology, Biochemical Testing)
   • Antibiotic Susceptibility Testing
   • PCR-Based Identification Methods

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Part II: Genetic and Molecular Techniques

3. Genomic DNA Extraction and Purification

   • Methods for Extracting DNA from Proteus mirabilis
   • Quality Control of DNA Samples
   • Protocols for RNA Extraction (if applicable)

4. Cloning and Transformation Techniques

   • Protocols for Cloning Plasmids in Proteus mirabilis
   • Electroporation and Chemical Transformation Methods

5. Quantitative PCR and Gene Expression Analysis

   • Protocol for Quantitative PCR (qPCR) in Proteus mirabilis
   • Techniques for Measuring Gene Expression
   • Analysis of Virulence Factors

6. Gene Knockdown and Gene Editing

   • RNA Interference Techniques
   • CRISPR/Cas9-based Gene Editing in Proteus mirabilis

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Part III: Biofilm Formation and Virulence Studies

7. Biofilm Formation Assays

   • Crystal Violet Assay for Biofilm Quantification
   • Flow Cell and Microplate Assays
   • Confocal Microscopy for Biofilm Imaging

8. Virulence Factor Analysis

   • Hemolysin Production and Assays
   • Urease Activity Assays
   • Swarming Motility Analysis
   • Flagella and Pili Formation

9. Animal Models for Proteus mirabilis Infection

   • Mouse UTI Models
   • In Vivo Assessment of Virulence
   • Kidney Stone Formation in Animal Models

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Part IV: Molecular Interactions and Pathogenesis

10. Host-Bacterial Interaction Studies

• Cell Culture Models for Studying Infection
• Epithelial Cell Adhesion Assays
• Immune Response Modulation by Proteus mirabilis

11. Proteomic and Metabolomic Profiling

• Mass Spectrometry for Protein Identification
• Techniques for Studying Metabolic Pathways in Proteus mirabilis

12. Antibiotic Resistance and Mechanisms of Resistance

• Mechanisms of Resistance in Proteus mirabilis
• Methods for Assessing Antibiotic Resistance
• Efflux Pump Activity and Inhibitor Screening

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Part V: Advanced Topics and Techniques

13. Advanced Imaging Techniques

• Fluorescence Microscopy for Studying Proteus mirabilis Behavior
• Electron Microscopy for Biofilm Visualization
• Live-Cell Imaging and Time-Lapse Studies

14. High-Throughput Screening and Omics Approaches

• High-Throughput Screening for Virulence Factors
• Transcriptomics and Metabolomics Protocols for Proteus mirabilis
• Proteomics in Infection Studies

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