Reverse Genetics of RNA Viruses Methods and Protocols Methods in Molecular Biology 2733 2nd Edition by Daniel R Perez ISBN 1071635328 9781071635322

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ISBN 10: 1071635328 
ISBN 13: 9781071635322
Author: Daniel R Perez

This second edition volume expands on the previous edition with new and updated chapters that highlight the latest methods and approaches for the manipulation of RNA viruses. The chapters in this book explore the fundamental role in studying RNA viruses; identifying markers of host range, disease, and transmission; and aid readers with the further development of in silico computational biology tools and artificial intelligence algorithms that can help predict the emergence of certain pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Reverse Genetics of RNA Viruses: Methods and Protocols, Second Edition is a valuable resource for researchers who are interested in learning more about this developing field.

Reverse Genetics of RNA Viruses Methods and Protocols Methods in Molecular Biology 2733 2nd Table of contents:

• Part I: General Considerations in Reverse Genetics of RNA Viruses

  • Chapter 1: Reverse Genetics and Its Applications in Virology
  • Chapter 2: Considerations for RNA Virus Reverse Genetics
  • Chapter 3: Key Tools in Reverse Genetics of RNA Viruses

• Part II: Reverse Genetics of Positive-Strand RNA Viruses

  • Chapter 4: Reverse Genetics of Picornaviruses
  • Chapter 5: Reverse Genetics of Coronaviruses
  • Chapter 6: Reverse Genetics of Flaviviruses
  • Chapter 7: Reverse Genetics of Togaviruses
  • Chapter 8: Reverse Genetics of Astroviruses and Hepeviruses

• Part III: Reverse Genetics of Negative-Strand RNA Viruses

  • Chapter 9: Reverse Genetics of Rhabdoviruses
  • Chapter 10: Reverse Genetics of Paramyxoviruses
  • Chapter 11: Reverse Genetics of Orthomyxoviruses (Influenza Virus)
  • Chapter 12: Reverse Genetics of Filoviruses
  • Chapter 13: Reverse Genetics of Bunyaviruses and Arenaviruses

• Part IV: Reverse Genetics of Other RNA Viruses

  • Chapter 14: Reverse Genetics of Reoviruses
  • Chapter 15: Reverse Genetics of Retroviruses
  • Chapter 16: Reverse Genetics of Hepatitis C Virus

• Part V: Advanced Techniques and New Developments in Reverse Genetics

  • Chapter 17: RNA Editing and Reverse Genetics
  • Chapter 18: CRISPR/Cas9 Technology in RNA Virus Research
  • Chapter 19: Next-Generation Sequencing and Reverse Genetics
  • Chapter 20: In Vivo Reverse Genetics and Animal Models

• Part VI: Applications of Reverse Genetics in Vaccine Development and Therapeutics

  • Chapter 21: Reverse Genetics for Vaccine Development
  • Chapter 22: Reverse Genetics and Antiviral Drug Discovery
  • Chapter 23: Reverse Genetics in the Development of Oncolytic Viruses

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Daniel R Perez,Reverse Genetics,RNA Viruses,Protocols,Molecular Biology