Rheumatoid Arthritis Methods and Protocols Methods 2nd Edition by Shuang Liu 1071636812 9781071636817

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ISBN-10 :  1071636812 

ISBN-13 :  9781071636817

Author:  Shuang Liu 

This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Rheumatoid Arthritis Methods and Protocols Methods 2nd Table of contents:

1. Collagen-induced arthritis models.
2. Human xenograft model.
3. Scaffolded chondrogenic spheroid engrafted model.
4. Denervation-induced sarcopenia model.
5. Long-term constant subcutaneous drug administration.
6. Clinical Scoring of Disease Activity in Animal Models.
7. Histological analyses of arthritic joints in collagen-induced arthritis model mice.
8. Preparation of Joint Extracts.
9. Production of immunizing antigen proteoliposome using cell-free protein synthesis system.
10. Re-construction of protein/liposome complex.
11. Production of neutralizing antibody.
12. Autoantibody profiling using human autoantigen protein array and AlphaScreen.
13. Generation of Specific Aptamers.
14. Detailed Protocol for Predicting 3D Structure of DNA Aptamers and Performing In Silico Docking Calculations.
15. RNA interference ex vivo.
16. Lentiviral-mediated systemic RNA interference in vivo.

 

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Tags: Rheumatoid Arthritis, Methods, Protocols Methods, Shuang Liu