Tandem Mass Spectrometry of Lipids Molecular Analysis of Complex Lipids 1st Edition by Robert C Murphy ISBN 1782626352 9781782626350

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ISBN 10: 1782626352
ISBN 13: 9781782626350
Author: Robert C Murphy

The emerging field of lipidomics has been made possible because of advances in mass spectrometry, and in particular tandem mass spectrometry of lipid ions generated by electrospray ionization. The ability to carry out basic biochemical studies of lipids using electrospray ionization is predicated upon understanding the behaviour of lipid derived ions following collision induced decomposition and mechanisms of product ion formation. During the past 20 years, a wealth of information has been generated about lipid molecules that are now analysed by mass spectrometry, however there is no central source where one can obtain basic information about how these very diverse biomolecules behave following collisional activation.

This book brings together, in one volume, this information so that investigators considering using tandem mass spectrometry to structurally characterize lipids or to quantitate their occurrence in a biological matrix, will have a convenient source to review mechanism of decomposition reactions related to the diversity of lipid structures.

A separate chapter is devoted to each of seven major lipid classes including fatty acids, eicosanoids and bioactive lipid mediators, fatty acyl esters and amides, glycerol esters, glycerophospholipids, sphingolipids, and steroids. Mechanistic details are provided for understanding the pathways of formation of major product ions and ions used for structural characterization. In most cases specific ancillary information has been critical to understand the pathways, including isotope labeling and high resolution analysis of precursor and product ions. For a few specific examples such data is missing and pathways are proposed as a means to initiate further mass spectral experiments to prove or disprove pathway hypotheses. While this work largely centres on the lipid biochemistry of animal (mammalian) systems, general principles can be taken from the specific examples and applied to lipid biochemistry found in plants, fungi, prokaryotes and archeal organisms.

Tandem Mass Spectrometry of Lipids Molecular Analysis of Complex Lipids 1st Table of contents:

1. Introduction to Lipidomics

   • Overview of Lipidomics and Its Significance
   • Lipid Classification and Nomenclature
   • Role of Lipids in Biological Systems
   • Challenges in Lipid Analysis

2. Fundamentals of Mass Spectrometry

   • Introduction to Mass Spectrometry
   • Basic Principles of Mass Spectrometry
   • Tandem Mass Spectrometry (MS/MS)
   • Ionization Techniques for Lipid Analysis
   • Data Acquisition and Interpretation

3. Lipid Extraction and Sample Preparation

   • Methods of Lipid Extraction from Biological Samples
   • Sample Preparation Techniques for Mass Spectrometry
   • Contamination Control and Standardization

4. Lipid Profiling Using Mass Spectrometry

   • Lipid Profiling Techniques
   • Analysis of Major Lipid Classes (Phospholipids, Sphingolipids, Glycolipids, etc.)
   • Lipid Identification and Quantification

5. Tandem Mass Spectrometry of Phospholipids

   • Overview of Phospholipid Structure
   • Mass Spectrometric Analysis of Phospholipids
   • Fragmentation Patterns in Phospholipids
   • Applications in Cellular and Tissue Lipid Analysis

6. Analysis of Sphingolipids Using Tandem Mass Spectrometry

   • Sphingolipid Class and Structure
   • Mass Spectrometric Techniques for Sphingolipid Analysis
   • Key Fragmentation Mechanisms in Sphingolipid MS/MS

7. Glycolipids and Other Complex Lipids

   • Glycolipids and Their Biological Role
   • Mass Spectrometric Analysis of Glycolipids
   • Tandem MS for Analysis of Complex Glycolipids

8. Lipid Mapping and Imaging

   • Techniques for Lipid Mapping Using Mass Spectrometry
   • Imaging Mass Spectrometry for Lipid Localization
   • Applications in Tissue and Organ Lipid Distribution

9. Quantitative Lipidomics

   • Quantification of Lipid Species Using Mass Spectrometry
   • Calibration and Internal Standards in Lipid Analysis
   • High-Throughput Lipid Quantification Methods

10. Data Analysis and Interpretation in Lipidomics

    • Data Processing and Interpretation in Mass Spectrometry
    • Lipid Identification Algorithms and Databases
    • Statistical Analysis in Lipidomics Studies

11. Applications of Tandem Mass Spectrometry in Lipid Research

    • Lipid Signaling Pathways and Mass Spectrometry
    • Lipids in Disease Research (Cancer, Neurodegeneration, etc.)
    • Clinical Applications of Lipidomics

12. Emerging Trends in Lipid Mass Spectrometry

    • Advances in Mass Spectrometry Techniques for Lipidomics
    • The Role of High-Resolution MS in Lipid Analysis
    • Future Directions in Lipidomics and Mass Spectrometry

13. Conclusion

    • Summary of Key Techniques and Applications
    • Challenges and Opportunities in Lipid Mass Spectrometry
    • The Future of Lipidomics

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