The Laboratory Mouse: A Guide to the Location and Orientation of Tissues for Optimal Histological Evaluation 1st Edition Jennifer 0429614144 9780429614149

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Product details:

• ISBN 10: 0429614144
• ISBN 13: 9780429614149
• Author: Jennifer Johnson

Key features: High quality full color photographs and descriptive texts on the location and removal of the organs from the mouse Instructive methods and clear visuals for trimming and orienting the organs for paraffin histology to obtain the best possible sections for analysis Full color photomicrographs of the resulting section for each organ stained with hematoxylin and eosin demonstrating important features and landmarks for the histologist to ensure the optimal area for analysis is achieved All in one, easy to use guide organized by individual organs of the laboratory mouse Spiralbound for easy reference in the lab This “one-stop” guide offers an essential resource for any academic, research or development operation where mouse necropsy and/or histology are performed. Connecting the reader ‘from the mouse to the microscope’, it provides a detailed guide for locating, trimming, orientating and embedding of the most frequently investigated tissues collected in the laboratory mouse. It shows where the organs reside in the mouse, how to trim and embed them as well as the resulting optimal sections. This guide brings together the wealth of scattered information into one high-quality text, the emphasis is on providing knowledge that will help histologists and scientists get better results in any downstream assays where ideal sections are needed.

Table of contents:

Adrenal Glands
Brain
Brain – Trimming for Coronal Sections
Brain – Trimming for Sagittal Sections
Diaphragm
Esophagus, Trachea and Thyroid
Eyes
Female – Ovaries, Oviducts
Female – Uterus (Uterine Horn), Cervix, Vagina
Femur
Heart
Kidneys
Liver and Gallbladder
Lung (Inflated)
Lymph Nodes – Axillary
Lymph Nodes – Mesenteric
Male – Epididymis
Male – Preputial Gland
Male – Seminal Vesicle, Coagulating Gland, Prostate Gland
Male – Testes
Pancreas
Pituitary Gland
Quadriceps Muscle
Salivary Glands
Sciatic Nerve
Skin with (or without) Mammary Gland
Spinal Cord
Spine
Spleen
Sternum
Stomach – Open Method
Stomach – Whole Method
Stifle Joint
Thymus
Tongue
Urinary Bladder
Intestines
Small Intestine – Duodenum
Small Intestine – Jejunum
Small Intestine – Ileum
Large Intestine – Cecum
Large Intestine – Colon
Large Intestine – Rectum
Materials and Methods

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