The Plant Endoplasmic Reticulum Methods and Protocols 1st Edition by Chris Hawes, Verena Kriechbaumer 1493973886 978-1493973880

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Product details:

ISBN 10: 1493973886

ISBN 13: 978-1493973880

Author: Chris Hawes, Verena Kriechbaumer

Table of contents:

1. Labelling the ER for light and fluorescence microscopy

2. Electron microscopy techniques for 3D imaging of plant ER

3. Studying plant ER–plasma membrane (PM) contact site–localized proteins using microscopy

4. Preparation and imaging of specialized ER using super-resolution and transmission electron microscopy (TEM)

5. Quantitation of ER morphology and dynamics

6. Long-term imaging of ER morphology in embryos during seed germination

7. “Dancing with the stars”: image analysis to study the choreography of the ER, its partners, and movement within its tubules

8. Preparation of highly enriched ER membranes using free-flow electrophoresis

9. Preparation of ER microsomes from Arabidopsis thaliana

10. ER membrane lipid composition and metabolism via lipidomic analysis

11. 2-in-1 vectors for improved in planta BiFC and FRET analysis

12. Immunoprecipitation and FRET-FLIM to identify metabolons on the plant ER

13. Using optical tweezers combined with total internal reflection microscopy (TIRF) to study ER–Golgi interactions in plant cells

14. Protein biosynthesis and maturation in the ER

15. ER membrane protein interactions studied using the Split-Ubiquitin System (SUS)

16. Analysis of protein glycosylation in the ER

17. Unfolded Protein Response (UPR) in Arabidopsis

18. Imaging the ER and endomembrane system in cereal endosperm

19. Multi-omics resources for understanding gene regulation in response to ER stress in plants

20. Variable-angle epifluorescence microscopy for single-particle tracking in the plant ER

21. Super-resolution live-cell imaging to distinguish ER and nuclear envelope subcellular localization

22. Using ER-targeted photoconvertible fluorescent proteins in living plant cells

23. In vivo analysis of ER-associated protein degradation and ubiquitination in Arabidopsis thaliana

24. Overexpression of ER proteins from Arabidopsis thaliana using baculovirus expression systems

25. Observing ER dynamics over long timescales using light sheet fluorescence microscopy

26. Gene stacking and stoichiometric expression of ER-targeted constructs via “2A” self-cleaving peptides

27. Automated segmentation and 3D reconstruction of different membranes from confocal Z-stacks

28. Visualizing orientation and topology of ER membrane proteins in planta

29. Organ-specific microsomes from dark-grown hypocotyls of Arabidopsis thaliana

30. Studying secretory protein synthesis in Nicotiana benthamiana protoplasts

31. Analyzing photoactivation with diffusion models to study transport within the ER network

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Tags: Chris Hawes, Verena Kriechbaumer, Endoplasmic, Methods