The Plant Microbiome Methods and Protocols 1st Edition by Lilia Carvalhais, Paul Dennis ISBN 1071610392 9781071610398

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Product details:
ISBN 10: 1071610392
ISBN 13: 9781071610398
Author: Lilia Carvalhais, Paul Dennis

This volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based methods, culture-independent metabarcoding methods, methods to obtain DNA and perform metabarcoding, protocols to block PCR amplification from the plant host, qPCR-based methods, editing of specific genes in Bacillus genomes, and Streptomycetes and plant microbial indicators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Plant Microbiome: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The Plant Microbiome: Methods and Protocols 1st Edition Table of contents:

• Chapter 1: Sampling and Isolation of Plant Microbiomes

  • Techniques for sampling plant tissues and environments
  • Methods for isolating microbial communities from plants
  • Considerations for sample handling and preservation

• Chapter 2: Cultivation and Enrichment of Plant-associated Microorganisms

  • Cultivation techniques for isolating plant-associated microbes
  • Enrichment cultures for specific microbial groups
  • Protocols for growing and maintaining microbial cultures

• Chapter 3: DNA Extraction from Plant Samples

  • Protocols for high-quality DNA extraction from plant tissues
  • Tips for preventing contamination
  • Techniques for extracting DNA from complex microbiomes

• Chapter 4: Metagenomic Approaches for Characterizing Plant Microbiomes

  • Introduction to metagenomics in plant microbiome research
  • Sequencing techniques and technologies
  • Data analysis methods for metagenomic studies

• Chapter 5: Amplicon Sequencing for Microbial Profiling

  • Using 16S rRNA and ITS markers to profile microbial communities
  • Library preparation for amplicon sequencing
  • Analyzing sequencing data to reveal microbial diversity

• Chapter 6: Functional Profiling of the Plant Microbiome

  • Methods for evaluating microbial functionality within plant-associated communities
  • Functional metagenomics and metatranscriptomics
  • Tools and approaches for annotating microbial functions

• Chapter 7: Microbial Interaction Studies

  • Techniques for studying plant-microbe interactions in the laboratory
  • In vitro and in vivo assays for plant-microbe interaction research
  • Quantifying and characterizing plant responses to microbial communities

• Chapter 8: Understanding the Role of the Microbiome in Plant Stress Tolerance

  • Methods for studying how plant microbiomes mediate stress responses
  • Microbiome-induced resistance to pathogens and environmental stress
  • Protocols for experimental stress models in plants

• Chapter 9: Plant Microbiome in Sustainable Agriculture

  • The role of plant microbiomes in sustainable farming practices
  • Methods for enhancing soil health and plant growth through microbiomes
  • The potential for microbiome-based biofertilizers and biocontrol agents

• Chapter 10: Future Directions in Plant Microbiome Research

  • Emerging technologies in microbiome analysis
  • The future of microbiome-based solutions in agriculture
  • Challenges and opportunities in plant microbiome research

• Appendices

  • Additional resources, troubleshooting tips for protocols
  • List of common tools and reagents
  • Glossary of terms

• Index

  • A comprehensive index for quick reference of topics, terms, and techniques

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Lilia Carvalhais,Paul Dennis,Plant Microbiome,Methods,Protocols