Vaccine Delivery Technology Methods and Protocols 1st edition by Blaine Pfeifer, Andrew Hill 1071607947 9781071607947

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ISBN 10: 1071607947 

ISBN 13: 9781071607947

Author:  Blaine A. Pfeifer, Andrew Hill 

This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.

Vaccine Delivery Technology: Methods and Protocols 1st Table of contents: 

1. Vaccine Delivery and Immune Response Basics
2. Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes
3. Use of Chlamydial Elementary Bodies as Probes to Isolate Pathogen‐Specific Human Monoclonal Antibodies
4. Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed
5. Antigen Discovery in Bacterial Pan-Proteomes
6. Alphavirus-Based Antigen Preparation
7. Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells
8. Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System
9. High-Throughput Process Development for the Chromatographic Purification of Viral Antigens
10. Production of Zika Virus Virus-Like Particles
11. Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs)
12. Up- and Downstream Processes for Viral Nanoplexes as Vaccines
13. Whole-Cell Vaccine Preparation: Options and Perspectives
14. O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein
15. Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines
16. Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens Used in Glycoconjugate Vaccines
17. Attenuation Methods for Live Vaccines
18. Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism
19. Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells
20. Lipofection-Based Delivery of DNA Vaccines
21. Flavivirus DNA Vaccine Design and Adjuvant Selection
22. Vaccine Delivery with a Detoxified Bacterial Toxin
23. Needle-Less or Non-Invasive Delivery Technology
24. Lactic Acid Bacteria as Delivery Vehicle for Therapeutic Applications
25. A Hybrid Biological-Biomaterial Vector for Antigen Delivery
26. Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens
27. Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder
28. The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers
29. Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice
30. The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot
31. The Measurement of Antigen-Specific IgG Titers by Direct ELISA
32. Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model
33. Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils
34. Viral Infectivity Quantification and Neutralization Assays using Laser Force Cytology

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Tags: Vaccine Delivery, Technology, Methods, Protocols, Blaine Pfeifer, Andrew Hill